Meso Scale Discovery (MSD) provides bioanalytics solutions for complex biological assays. These solutions include valuable and cost-effective assays such as MSD cytokine and MSD multiplex ELISA assay. MSD assay services are based on a proprietary combination of electrochemiluminescence detection and multiarray technology, enabling multiplex detection with a wide dynamic range and exceptional sensitivity.
MSD assay services include a robust detection system, customized microplates and instruments, and proprietary assay reagents. Besides, they offer customized solutions to their customers. MSD multiarray plates come in 96, 384, and 1536 wells in regular and high-binding capacity formats. Moreover, these plates can be coated with avidin or uncoated and complemented with custom coatings and surface treatments. Hence, GLP bioanalytical labs have MSD assay as a crucial component in their bioanalytical arsenal. The current article is a comprehensive guide to understanding and optimizing MSD assay services results.
Understanding and Optimizing MSD Results
MSD assay work on the Discovery workbench data analysis toolbox. This toolbox contains methods for analyzing assay results. This system allows analyzing data after it’s collected or stored in the plate data history. The Discovery workbench has data analysis functions to assess complex data sets. The plate history database has all experiments stored along with the experimental data. Besides, the software employs a 4-parameter logistic curve and standards to calculate control and unknown concentrations. Moreover, graphs, tables, and reports can be efficiently transferred to Word, PDF, and Excel documents.
Change control and user tracking features are available in alterable formats for tracing experiments and protocols. Moreover, the data analysis toolbox can also be installed on the desktop in addition to MSD instruments for conveniently analyzing experimental data. Also, the experiment templates and layouts can be shared among different laboratory personnel.
The MSD work bench protocol includes five primary steps:
- Run assay plate
- Create layout
- Analyze data
- Modify tables
- Generate report
Alternately, after templates and layouts are created, researchers can select a specific template to create an experiment automatically. Once the plate layout is ready, researchers can quickly analyze the plate to assess sample distribution on assay plates.
The first step in understanding and optimizing assay results is constructing an experiment. One may create an experiment by highlighting a specific plate in the plate data history. Researchers can rename the experiment groups and plates by right-clicking on items and selecting rename. MSD software presents data results in plots and table formats. Besides, one may edit, shorten and customize tables and plots based on individual needs.
The next step is reporting and presenting experimental data. Resources can easily compile all the data with a single click. One may highlight and select a specific format. Besides, these reports can be individually customized by selecting sections that one may require to be included in the PDF report.
Moreover, researchers can also transfer current experiments into other software programs. Researchers can copy the table by opening the table first and then copying it to a clipboard to manipulate or modify that section. Similarly, one may copy a plot to the clipboard and transfer the information into another application. Moreover, the software also has shortcuts to copy data to the clipboard.
In conclusion, understanding and optimizing assay results is critical for unlocking all potentials of MSD bioanalytical solutions.